Journal: Data in Brief

Article Title: Data supporting the functional role of Eleven-nineteen Lysine-rich Leukemia 3 (ELL3) in B cell lymphoma cell line cells

doi: 10.1016/j.dib.2017.09.042

Figure Lengend Snippet: ELL family member transcript levels in murine primary B cells and human B cell lymphoma cell lines. A. Relative mRNA expression profile of ELL family members across human B cell lines. The cell line name and lymphoma subtype are indicated on the x-axis. Data represents the average of 3 independent experiments; errors bars represent SD. B. Expression of ELL family members mRNA in murine B cells during proliferation and differentiation in response to LPS stimulation in vivo. Data was extracted from GSE70294 and presented as mRNA copies per cell. The x-axis represents the number of cell divisions. CD138 positivity is indicated by the (+) and represents the fully differentiated plasma cells. Data represents one experiment with biological duplicates. * p <0.05, ** p <0.01 ( two-tailed t-test ).

Article Snippet: How data was acquired , – quantitative PCR (qPCR) (Bio-Rad CFX96 Real-Time PCR Detection System and CFX96 Software) – Reanalyzed publically available RNA-Seq experiment GSE70294 – Time lapse imaging (Evos Auto FL Cell Imaging System and Image Studio Software) – Western blot (SDS-Page gel electrophoresis and wet transfer; Bio-Rad equipment and Bio-Rad clarity chemiluminescent detection).

Techniques: Expressing, In Vivo, Clinical Proteomics, Two Tailed Test

Journal: STAR Protocols

Article Title: Co-immunoprecipitation and semi-quantitative immunoblotting for the analysis of protein-protein interactions

doi: 10.1016/j.xpro.2021.100644

Figure Lengend Snippet:

Article Snippet: Wet-blot transfer equipment , Bio-Rad , 1703930.

Techniques: Virus, Recombinant, Protease Inhibitor, Modification, Software, Cell Culture, Control, Cell Counting, Purification, Electrophoresis, Nucleic Acid Electrophoresis, Imaging, Fluorescence, Microscopy, Blocking Assay, Membrane, Pore Size, Western Blot, Sterility